Which HDL quantitation method is non-chromatographic?

Which HDL quantitation method is non-chromatographic?

• A. Gomori procedure
• B. Homogeneous
• C. Column chromatography
• D. Agarose gel electrophoresis

Answer: (B)

Non-chromatographic HDL quantitation means measuring HDL cholesterol without using a chromatography step to separate HDL from other lipoproteins. The homogeneous HDL assay achieves this in one in-solution step: reagents selectively mask non-HDL interference and produce a signal that reflects just the HDL cholesterol present, so no physical separation is needed. Because there’s no column, gel, or precipitation-driven separation involved, it’s considered non-chromatographic. Other options involve separation or precipitation steps (column chromatography, electrophoresis, or precipitation-based methods), which are different approaches and not the direct in-solution measurement that defines the homogeneous method.

Non-chromatographic HDL quantitation means measuring HDL cholesterol without using a chromatography step to separate HDL from other lipoproteins. The homogeneous HDL assay achieves this in one in-solution step: reagents selectively mask non-HDL interference and produce a signal that reflects just the HDL cholesterol present, so no physical separation is needed. Because there’s no column, gel, or precipitation-driven separation involved, it’s considered non-chromatographic. Other options involve separation or precipitation steps (column chromatography, electrophoresis, or precipitation-based methods), which are different approaches and not the direct in-solution measurement that defines the homogeneous method.