Which HDL method uses gel electrophoresis to separate lipoproteins?

Which HDL method uses gel electrophoresis to separate lipoproteins?

• A. Gomori procedure
• B. Homogeneous
• C. Column chromatography
• D. Agarose gel electrophoresis

Answer: (D)

Lipoprotein separation by a gel matrix is the key idea here. In agarose gel electrophoresis, particles move through a porous gel under an electric field, and their rate of migration depends on size and surface charge. Because HDL, LDL, and other lipoproteins have different compositions and charges, they migrate to distinct positions and form separate bands. This is why agarose gel electrophoresis is the method that uses gel-based separation of lipoproteins. The other options rely on precipitation or chromatography rather than a gel, so they don’t employ a gel matrix to separate the lipoproteins.

Lipoprotein separation by a gel matrix is the key idea here. In agarose gel electrophoresis, particles move through a porous gel under an electric field, and their rate of migration depends on size and surface charge. Because HDL, LDL, and other lipoproteins have different compositions and charges, they migrate to distinct positions and form separate bands. This is why agarose gel electrophoresis is the method that uses gel-based separation of lipoproteins. The other options rely on precipitation or chromatography rather than a gel, so they don’t employ a gel matrix to separate the lipoproteins.