Which bilirubin measurement method is described as the most widely used?

Which bilirubin measurement method is described as the most widely used?

• A. Jaffe reaction
• B. Schales and Schales method
• C. 8-Hydroxyquinoline reaction
• D. Jendrassik-Grof method

Answer: (D)

The most widely used bilirubin measurement method is the diazo-based two-reagent approach that forms a stable color for spectrophotometric reading. This Jendrassik-Grof method became dominant because it delivers reliable and accurate results quickly, and it works well with automated analyzers, making it practical for routine clinical use. The key is that it completes the color development for both direct (conjugated) and indirect (unconjugated) bilirubin under conditions that minimize interference from substances in blood samples, producing a stable, easily measured color. Older or less robust options exist for bilirubin measurement. The Jaffe reaction is actually a different test (used for creatinine), not bilirubin. The Schales and Schales method is an earlier bilirubin method that can be more susceptible to interference from other pigments and sample components. The eight-hydroxyquinoline method is less commonly used and generally not as robust or widely adopted as the Jendrassik-Grof approach.

The most widely used bilirubin measurement method is the diazo-based two-reagent approach that forms a stable color for spectrophotometric reading. This Jendrassik-Grof method became dominant because it delivers reliable and accurate results quickly, and it works well with automated analyzers, making it practical for routine clinical use. The key is that it completes the color development for both direct (conjugated) and indirect (unconjugated) bilirubin under conditions that minimize interference from substances in blood samples, producing a stable, easily measured color.

Older or less robust options exist for bilirubin measurement. The Jaffe reaction is actually a different test (used for creatinine), not bilirubin. The Schales and Schales method is an earlier bilirubin method that can be more susceptible to interference from other pigments and sample components. The eight-hydroxyquinoline method is less commonly used and generally not as robust or widely adopted as the Jendrassik-Grof approach.